MHC PEPTIDOMICS

MHC PEPTIDOMICS

All nucleated cells in higher vertebrates express MHC class I proteins on their surface (HLA class I in humans), while only a subset of cells (mostly professional antigen-presenting cells) express MHC class II.
Malignant cells, which display tumor-associated peptides on MHC molecules, can be recognized and killed by T cells. There is a growing evidence that the tumor rejection process can be boosted by suitable immunostimulatory approaches, such as treatment with anti-PD-1 antibodies or immunocytokines.

Philochem scientists have developed powerful methodologies for the detailed analysis of human and mouse MHC-bound peptides. Indeed, thousands of MHC class I and MHC class II bound peptides can be identified with high confidence, using a combination of immunocapture and state-of-the-art mass spectrometry. Once the tumor- or disease-associated peptides have been identified, they can be incorporated in artificial MHC tetramers to test their immunoreactivity. Insights into T cell recognition processes are invaluable for the development of immunomodulatory strategies against cancer and chronic inflammatory conditions. Furthermore, MHC peptidome analysis provides useful biomarkers for patient stratification strategies.
 

SELECTED REFERENCES

Probst et al. (2019) Clin Cancer Res, 25, 698-709
Probst et al. (2017) Cancer Res, 77, 3644-54
Ritz et al. (2017) Proteomics, 17, 10.1002/pmic.201600364
Gloger et al. (2016) Cancer Immunol Immunother, 65, 1377-93
Ritz et al. (2016) Proteomics, 16, 1570-80
Sofron et al. (2016) Eur J Immunol, 46, 319-28
 

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