All nucleated cells in higher vertebrates express MHC class I proteins on their surface (HLA class I in humans), while only a subset of cells (mostly professional antigen-presenting cells) MHC PEPTIDOMICS
Figure 1HLA class I-eluted peptides from HEK293 cells reveal their HLA-specific motifs. The methodology can be used for the analysis of cell cultures, of serum samples and of tissue lysates.
express MHC class II. Malignant cells, which display certain "tumor rejection antigens" on MHC molecules, can be recognized and killed by T cells. There is a growing evidence that the tumor rejection process can be boosted by suitable immunostimulatory approaches, such as treatment with anti-PD-1 antibodies or immunocytokines.
Philochem scientists have developed powerful methodologies for the detailed analysis of human and mouse MHC-bound peptides. Indeed, thousands of MHC class I and MHC class IIbound peptides can be identified with high confidence, using a combination of immunocapture and state-of-the-art mass spectrometry. Insights gained in the T cell recognition process are invaluable for the development of immunomodulatory strategies against cancer and chronic inflammatory conditions. Furthermore, MHC peptidome analysis provides useful biomarkers for patient stratification strategies.
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Sofron et al. (2016) Eur. J. Immunol. 46:319-328.